Skip to Content
MilliporeSigma

HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem

HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

Guard cartridge

Product No.
Description
Pricing

Zenix® SEC-300 Gel Filtration Guard Column, 3 μm

L × I.D. 5 cm × 7.8 mm, 3 μm particle size, stainless steel

CONDITIONS

column

Zenix SEC-300 (2 ea), 30 cm x 7.8 mm I.D., 3 μm partcles, 300 Å, connected in tandem (Z777033)

mobile phase

150 mM Sodium Phosphate Buffer pH=7.0

flow rate

0.5 mL/min

column temp.

25 °C

detector

Refractive Index at 30 °C

injection

10 μL

sample

1.PEG 81,400 Da (2mg/mL)

2.PEG 50,630 Da (2mg/mL)

3.PEG 40,000 Da (2mg/mL)

4.PEG 26,100 Da (2mg/mL)

5.PEG 8,730 Da (2mg/mL)

Description

General description

Pegylation of biotherapeutic drugs improves solubility, increases half life in the blood stream and reduces immunogenicity. Size exclusion chromatography (SEC) is often applied to characterize protein–polyethylene glycol (PEG) conjugates and establish the number of attached PEG chains, since SEC is a precise and robust technique that combines ease of set up and strainghtforward method develpopment.
This chromatogram shows the separation of four polyethyleneglycol polymers varying in molecular mass 8730 to 81,400 Da on two Zenix® SEC-300 columns in series.

Legal Information

Zenix is a registered trademark of Sepax Technologies