Skip to Content
MilliporeSigma

GC Analysis of Resveratrol in Red Wine (Spiked) on SLB®-5ms after SPME using a 85 μm Polyacrylate Fiber

GC Analysis of Resveratrol in Red Wine (Spiked) on SLB®-5ms after SPME using a 85 μm Polyacrylate Fiber suitable for GC, application for SPME

Materials

Analytical column

Product No.
Description
Pricing

SLB®-5ms Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 0.25 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly polyacrylate (PA)

df 85 μm(PA, for use with manual holder, needle size 24 ga

SPME fiber assembly polyacrylate (PA)

df 85 μm(PA, for use with autosampler, needle size 24 ga

SPME fiber assembly polyacrylate (PA)

df 85 μm(PA, for use with autosampler, needle size 23 ga

Standard

Product No.
Description
Pricing

Resveratrol

≥99% (HPLC)

accessory

Product No.
Description
Pricing

Vials, screw top with phenolic open-top cap, pre-assembled

volume 4 mL, clear glass vial, O.D. × H 15 mm × 45 mm, tan PTFE/silicone septum

related product

Product No.
Description
Pricing

SPME Fiber Holder

for use with manual sampling

SPME Fiber Holder

for use with CTC CombiPAL and Gerstel MPS2

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

pkg of 1 ea

CONDITIONS

sample/matrix

3 mL of red wine (California merlot) spiked with resveratrol at 100 μg/L then diluted 3:1 in 12% ethanol:water

SPME fiber

85 μm polyacrylate (57304, 57305, 57294-U)

extraction

immersion for 15 min at room temperature while stirring at 400 rpm, fiber gently blotted with a Kimwipe to remove excess water, (then derivatization by immersion in a 4 mL vial containing 5 μL of Sylon-BFT (fresh vial for each analysis than was allowed to equilabrate 60-90 minutes prior to use) for 20 min)

desorption process

280 °C for 2 min

column

SLB-5ms; 30 m x 0.25 mm I.D., 0.25 μm (28471-U)

oven

100 °C (1 min), 10 °C/min to 325 °C (3 min)

inj. temp.

280 °C

detector

GC/MS

MSD interface

325 °C

scan range

m/z 40-450

carrier gas

helium, 1 mL/min, constant

liner

0.75 mm I.D., SPME

sample

Red wine

Description

Analysis Note

In this study, the extraction and analysis of resveratrol from red wine is demonstrated using solid phase microextraction (SPME) and GC/MS. The presence of three hydroxyl (OH) groups makes it necessary to derivatize this compound prior to GC analysis. Derivatization was conducted, after extraction, directly on the SPME fiber by exposing it to the vapors of a silyating reagent. The derivative of resveratrol was then analyzed directly from the fiber using GC/MS. The polyacrylate fiber, which is suitable for the extraction of polar semivolatiles and is more resistant to swelling than other types of fibers was used in the analysis. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.

Legal Information

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany