HPLC Analysis of Antihistamines on Discovery^® C8

column

Discovery C8, 15 cm × 4.6 mm I.D., 5 μm particles (59353-U)

mobile phase

[A] acetonitrile; [B] 25 mM potassium phosphate, pH 2.3; (8:92, A:B)

flow rate

2 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

2 μL