Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
25 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid)
1 mL/min
35 °C
UV, 230 nm
10 μL
as indicated each in mobile phase
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC