MilliporeSigma

HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide

HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

standard

Product No.
Description
Pricing

Acetylsalicylic acid

analytical standard

Salicylic acid

BioXtra, ≥99.0%

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)

flow rate

1 mL/min

pressure

2200 psi (152 bar)

column temp.

30 °C

detector

UV, 250 nm

injection

5 μL

sample

50 μg/mL in 75:25, water:acetonitrile

Description

Analysis Note

Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany