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HPLC Analysis of Benzodiazepines on Ascentis® Express C18, Acetonitrile Mobile Phase

HPLC Analysis of Benzodiazepines on Ascentis® Express C18, Acetonitrile Mobile Phase application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

Product No.
Description
Pricing

Desmethyldiazepam

analytical standard

Oxazepam solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Alprazolam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Clonazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Diazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water; [B] acetonitrile; (66:34, A:B)

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 250 nm

injection

2 μL

sample

0.1 g/L each in 20% methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany