HPLC Analysis of Cardiac Drugs on Ascentis^® C8

column

Ascentis C8, 15 cm x 2.1 mm I.D., 3 μm particles (581402-U)

mobile phase

0.1% ammonium acetate:acetonitrile with 0.1% ammonium acetate, (47:53)

flow rate

0.2 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

2 μL

sample

as indicated in mobile phase