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HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide

HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

CONDITIONS

column

Ascentis RP-Amide, 5 cm × 4.6 mm I.D., 5 μm particles (565323-U)

mobile phase

[A] water with 0.1% ammonium formate (pH 3.04 with formic acid), [B] acetonitrile

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 260 nm

injection

5 μL

sample

as indicated in mobile phase A

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany