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HPLC Analysis of HPLC Protein Standard on BIOshell A400 Protein C4

HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

BIOshell A400 Protein C18 Column

L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

standard

Product No.
Description
Pricing

HPLC protein standard mixture

analytical standard

CONDITIONS

column

BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)

mobile phase

[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75

flow rate

0.3 mL/min

gradient

0 to 75% B in 15 min

pressure

2800 psi (193 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

2 μL

sample

ribonuclease A, cytochrome c, holo-transferrin and myoglobin 1 mg in mobile phase A

Description

Analysis Note

Analysis of protein standard of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC