HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4
CONDITIONS
column
BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)
mobile phase
[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
flow rate
0.3 mL/min
gradient
0 to 75% B in 15 min
pressure
2800 psi (193 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
2 μL
sample
ribonuclease A, cytochrome c, holo-transferrin and myoglobin 1 mg in mobile phase A
Description
Analysis Note
Analysis of protein standard of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
Legal Information
BIOshell is a trademark of Sigma-Aldrich Co. LLC