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HPLC Analysis of Nucleosides on Ascentis® Express HILIC

HPLC Analysis of Nucleosides on Ascentis® Express HILIC application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express HILIC, 10 cm × 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with concentrated formic acid: [B] acetonitrile; (10:90, A:B)

flow rate

0.4 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany