MilliporeSigma

HPLC Analysis of Nucleosides on Ascentis® Phenyl

HPLC Analysis of Nucleosides on Ascentis® Phenyl application for HPLC

Materials

standard

Product No.
Description
Pricing

Nucleosides Test Mix

in 1% sodium formate (varied conc.), analytical standard

Adenosine

Pharmaceutical Secondary Standard; Certified Reference Material

Disodium Guanylate

Pharmaceutical Secondary Standard; Certified Reference Material

Uridine

Pharmaceutical Secondary Standard; Certified Reference Material

Guanosine

Pharmaceutical Secondary Standard; Certified Reference Material

5′-Adenylic Acid

Pharmaceutical Secondary Standard; Certified Reference Material

Zidovudine Related Compound D

pharmaceutical secondary standard

Cytidine

certified reference material, pharmaceutical secondary standard

CONDITIONS

column

Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 μm particles (581613-U)

mobile phase

10 mM ammonium formate (pH 3.0 with formic acid)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell

injection

1 μL

sample

as indicated, in mobile phase

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany