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HPLC Analysis of Nucleotides on SUPELCOSIL LC-18-T

HPLC Analysis of Nucleotides on SUPELCOSIL™ LC-18-T application for HPLC

Materials

analytical column

Product No.
Description
Pricing

SUPELCOSIL LC-18-T HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

SUPELCOSIL LC-18-T, 15 cm × 4.6 mm I.D., 3 μm particles (58970-U)

mobile phase

[A] 0.1M potassium phosphate buffer/4mM tertrabutylammonium hydrogen sulfate, pH6.0; [B] A:methanol (70:30), pH 7.2

flow rate

1.5 mL/min

detector

UV, 254 nm

injection

20 μL deionized water (25-530 μM each analyte)

Description

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC