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HPLC Analysis of Organic Acids Commonly Found in Wine and Beer on TSKgel® OApak-P and OApak-A

HPLC Analysis of Organic Acids Commonly Found in Wine and Beer on TSKgel® OApak-P and OApak-A application for HPLC

Materials

analytical column

Product No.
Description
Pricing

TSKgel® OApak-A HPLC Column

phase carboxylic acid, L × I.D. 30 cm × 7.8 mm, 7 μm particle size

guard column

Product No.
Description
Pricing

TSKgel® OApak-P Guard Column

phase sulphonic acid, L × I.D. 4 cm × 6 mm, 10 μm particle size

mobile phase component

Product No.
Description
Pricing

Sulfuric acid concentrate

0.1 M H2SO4 in water (0.2N), eluent concentrate for IC

standard

Product No.
Description
Pricing

L-Pyroglutamic acid

≥99.0% (T)

Citric acid

United States Pharmacopeia (USP) Reference Standard

Lactic acid

United States Pharmacopeia (USP) Reference Standard

Malic acid

United States Pharmacopeia (USP) Reference Standard

Succinic acid

United States Pharmacopeia (USP) Reference Standard

Tartaric acid

United States Pharmacopeia (USP) Reference Standard

Pyruvic acid

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

TSKgel OApak-A, 30 cm x 7.8 mm I.D., 5 μm particles (816653)

column

TSKgel OApak-P Guard Column, 4 cm x 6 mm I.D., 10 μm particles (816654)

mobile phase

0.75 mM sulfuric acid

flow rate

0.8 mL/min

column temp.

60 °C

detector

conductivity

injection

20 μL

sample

organic acid mixture, see figure for concentrations

Description

Other Notes

Data provided by Tosoh Bioscience LLC.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation