MilliporeSigma

HPLC Analysis of Organic acids on Chromolith® Performance 100 - 4.6 mm

HPLC Analysis of Organic acids on Chromolith® Performance 100 - 4.6 mm application for HPLC

CONDITIONS

column

Chromolith® Performance, 100 x 4.6 mm (1.52028.0001)

mobile phase

[A] acetonitrile; [B] na-phosphatbuffer 20mM pH=2.5

gradient

Initial composition: 0-0.5min 100%B to 82%B, hold until 5min, 5-6min 82%B to 100%B, hold until 8.5min, 8.5-12min 100%B to 55%B, hold until 16min

flow rate

1 mL/min

pressure

940 psi (56-65 bar)

column temp.

ambient

detector

220 nm cell: 11 μL

injection

5 μL

sample

Malic acid (0,5mg/ml), Fumaric acid (0,15mg/ml), Glutaric acid (0,7mg/ml), 3,4-Dihydroxy-cinnamic acid (0,1mg/ml), 4-Hydroxy-cinnamic acid (0,1mg/ml), Sorbic acid (0,75mg/ml), Benzoic acid (0,2mg/ml), 2-Hydroxybenzoic acid (0,5mg/ml), Cinnamic acid (0,2mg/ml)

Description

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany