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HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Discovery® BIO Wide Pore C5 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)

mobile phase

[A]: 0.1% PFPA (pentafluoropropionic acid) in water:acetonitrile (81:19); [B]: 0.1% PFPA in water:acetonitrile (62:38)

gradient

0-100% B in 19 min

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

10 μL ~0.25 μg each peptide

sample

Cat. No. P2693

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany