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HPLC Analysis of Proteins (2) on BIOshell A400 Protein C4

HPLC Analysis of Proteins (2) on BIOshell A400 Protein C4 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

sample

protein mixture, g/L ea: superoxide dismutase, 0.3; bovine serum albumin, 0.3; alpha-lactalbumin, 0.2; beta-lactoglobulin A, 0.3; catalase, 0.3; (carbonic anhydrase, 0.3; beta-Amylase, 1.0; glucose-6-phosphate dehydrogenase, 1.0; thioredoxin, 0.3; phosphorylase b, 2.5.)

column

BIOshell A400 Protein C4, 10 cm X 2.1 mm I.D., 3.4 μm particles (66825-U)

mobile phase

[A] 75:25, (0.1% TFA in water):(0.1% TFA in acetonitrile) ; [B] 25:75, (0.1% TFA in water):(0.1% TFA in acetonitrile)

gradient

15 to 75% B in 10 min

flow rate

0.3 mL/min

pressure

2410 psi (166 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

1 μL

Description

Analysis Note

This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.

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