MilliporeSigma

HPLC Analysis of Proteins on BIOshell A400 Protein C18, Hydrophobicity Comparision

HPLC Analysis of Proteins on BIOshell A400 Protein C18, Hydrophobicity Comparision application for HPLC

Materials

analytical column

Product No.
Description
Pricing

BIOshell A400 Protein C18 Column

L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

standard

Product No.
Description
Pricing

Superoxide Dismutase from bovine erythrocytes

≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture

Carbonic Anhydrase from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

Catalase from bovine liver

lyophilized powder, ≥10,000 units/mg protein

β-Amylase from sweet potato

β-Lactoglobulin A from bovine milk

≥90% (PAGE)

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

α-Lactalbumin from bovine milk

Type III, calcium depleted, ≥85% (PAGE), lyophilized powder

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)

CONDITIONS

column

BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)

mobile phase

[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75

gradient

10 to 100% B in 18 min

flow rate

0.3 mL/min

pressure

2800 psi total pressure (193 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

5 μL

sample

superoxide dismutase, 0.13; a-lactalbumin, 0.13; serum albumin bovine, 0.13; ß-lactoglobulin A, 0.06; catalase, 0.13; (carbonic anhydrase, 0.13; ß-amylase, 0.13; glucose-6 phosphate dehydrogenase, 0.13 g/L in mobile phase A)

Description

Analysis Note

Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.

Legal Information

null