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HPLC Analysis of Ribonucleosides on Ascentis® Si

HPLC Analysis of Ribonucleosides on Ascentis® Si application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

Adenosine

Pharmaceutical Secondary Standard; Certified Reference Material

Uridine

Pharmaceutical Secondary Standard; Certified Reference Material

Guanosine

Pharmaceutical Secondary Standard; Certified Reference Material

Cytidine

certified reference material, pharmaceutical secondary standard

CONDITIONS

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 150 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 270 nm

injection

1 μL

sample

as indicated in mobile phase

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany