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HPLC Analysis of Sugars on SUPELCOSIL LC-NH2

HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

SUPELCOSIL LC-NH2 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

Maltose solution

BioReagent, for molecular biology, ~20% in H2O

Sucrose

suitable for microbiology, ACS reagent, ≥99.0%

Dextrose

United States Pharmacopeia (USP) Reference Standard

Fructose

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

SUPELCOSIL LC-NH2, 25 cm x 4.6 mm I.D., 5 μm particles (58338)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

1 mL/min

temp.

ambient

detector

refractive index (RI)

injection

2 μL

sample

10 mg/mL in water

Description

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC