Skip to Content
MilliporeSigma

HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano

HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® ES-Cyano HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

Amitriptyline hydrochloride solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-Desmethyldoxepin-D3 hydrochloride solution

cis/trans, 100 μg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Desipramine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Doxepin hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Imipramine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Nortriptyline hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Protriptyline hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis ES Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)

mobile phase

[A] 10 mM potassium phosphate dibasic, pH 7 with phosphoric acid; [B] acetonitrile; [C] methanol; (25:60:15, A:B:C)

flow rate

2 mL/min

column temp.

25 °C

detector

UV, 215 nm

injection

5 μL

sample

100 μg/mL in water: methanol (70:30)

Description

Analysis Note

This application demonstrates the suitability of Ascentis ES Cyano for the efficient separation of tricyclic antidepressants.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany