LC/MS Analysis of Amphetamines on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 10 cm x 3 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 1 mM ammonium acetate in [B] 10:90 (v/v) water:acetonitrile

flow rate

0.6 mL/min

pressure

1189 psi (82 bar)

column temp.

35 °C

detector

ESI(+), full scan, m/z 110-200

injection

2 μL

sample

10 mg/L each in acetonitrile