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LC/MS Analysis of Equine Performance-Enhancing Dopants GABA and AICAR on Ascentis® Express HILIC

LC/MS Analysis of Equine Performance-Enhancing Dopants GABA and AICAR on Ascentis® Express HILIC application for LC-MS

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

mobile phase component

Product No.
Description
Pricing

Ammonium formate

eluent additive for LC-MS, LiChropur, ≥99.0%

standard

Product No.
Description
Pricing

γ-Aminobutyric acid

≥99%

5-Hydroxy-L-tryptophan

powder

AICAR

≥98% (HPLC), powder

Ethyl-β-D-glucuronide

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 5 mM ammonium formate in acetonitrile:water (95:5); [B] 5 mM ammonium formate in acetonitrile:water (70:30)

gradient

0 to 70% B in 3 min, held for 1 min

flow rate

0.4 mL/min

column temp.

35 °C

detector

MS, ESI+/ESI-

injection

2 μL

sample

1 μg/mL in acetonitrile

Description

Analysis Note

This study focuses on the analysis of possible dopants used in equine racing, polar compounds requiring HILIC chromatographic separation. The rapid separation of four such compounds is shown here on an Ascentis Express HILIC column. The highest grade solvents provided clean, robust operation. Cerilliant ethyl-β-D-glucuronide CRM was used for reliable quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany