US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

Materials
Accessory
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Used Together
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CONDITIONS | |
|---|---|
| column | Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U) |
| mobile phase | [A] water; [B] acetonitrile |
| gradient | 10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min |
| flow rate | 1.0 mL/min |
| column temp. | 30 °C |
| detector | UV, 210 nm |
| injection | 10 μL |
| sample | as indicated in 16% acetonitrile in water |
Description | |
|---|---|
| Legal Information | Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany |
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