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MilliporeSigma

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

Materials

Accessory

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Used Together

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CONDITIONS

columnAscentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)
mobile phase[A] water; [B] acetonitrile
gradient10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min
flow rate1.0 mL/min
column temp.30 °C
detectorUV, 210 nm
injection10 μL
sampleas indicated in 16% acetonitrile in water

Description

Legal InformationAscentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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