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GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

GC Analysis of Terpenes in Hops (Cascade Variety) on Equity™-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber suitable for GC, application for SPME

Materials

analytical column

Product No.
Description
Pricing

Equity-1 Capillary GC Column

L × I.D. 60 m × 0.25 mm, df 0.25 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS)

needle size 23 ga, StableFlex, for use with autosampler

accessory

Product No.
Description
Pricing

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

pkg of 1 ea

Headspace vial, screw top, rounded bottom (vial only)

volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea

Magnetic Screw Cap for Headspace Vials, 18 mm thread

PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 ea

Molded Thermogreen® LB-2 Septa, with injection hole

diam. 11 mm, pkg of 50 ea

standard

Product No.
Description
Pricing

C7 - C40 Saturated Alkanes Standard

certified reference material, 1000 μg/mL each component in hexane

CONDITIONS

sample/matrix

ground Cascade variety hop pellets

SPME fiber

Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)

extraction

20 min, headspace, 40 °C

desorption process

3 min, 270 °C

sample preparation

30 min equilibration at 40 °C prior to extraction

sample preparation

fiber postbake after extraction, 3 min at 270 °C

column

Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)

oven

60 °C (2 min), 5 °C/min to 275 °C (5 min)

inj. temp.

270 °C

detector

MSD

MSD interface

300 °C

scan range

full scan, m/z 50-500

carrier gas

helium, 1 mL/min constant flow

liner

0.75 mm ID, SPME

Description

Analysis Note

Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in the Cascade variety of hops. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.

Other Notes

add 0.5 gm ground hops to a 10 mL headspace vial

Legal Information

Equity is a trademark of Sigma-Aldrich Co. LLC