UHPLC Analysis of Deuterated Isomers on Titan C18

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] water; [B] acetonitrile; (50:50, A:B)

flow rate

0.4 mL/min

pressure

5650 psi (390 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL