HPLC Analysis of Vitamin D2 and D3
Figure 1.Elution Order: Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)
Conditions | |
|---|---|
| column | Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 µm particles (50538-U) |
| column temp. | 35 °C |
| mobile phase | [A] acetonitrile; [B] methanol; (95:5, A:B) |
| flow rate | 1.5 mL/min |
| pressure | 1726 psi (119 bar) |
| sample | 50 µg/mL in 90:10, acetonitrile:methanol |
| injection | 10 µL |
| detector | UV, 265 nm |
Description | |
|---|---|
| Analysis Note | This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented. |
| Categories | Analytical Chromatography, Vitamins and Natural products, Steroids |
| Featured Industry | Clinical Vitamins, Nutraceuticals, and Natural Products |
| suitability | application for HPLC |
Materials
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