Product No. 3447-020-01
3-D Culture is an innovative approach to modeling the morphological effects of early oncogenesis on three-dimensional microenvironments. When healthy, differentiating cells exhibit a structured, polarized morphology that is critical for cellular formation and function. During carcinoma development, cell cycle controls associated with cellular development, proliferation and death are lost, and as a result, these structures are disrupted. In effect, the morphology of these structures can be used as a measure to study factors in early carcinoma development. In an attempt at standardization, J. Debnath, et al. published guidelines for execution of this assay using MCF-10A mammary epithelial cells as a model.1 The Cultrex® 3-D Culture Matrix™ product line provides reagents specifically produced for and qualified in 3-D culture studies. The 3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.
Type I Collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37 °C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.
Product is stable for a minimum of 3 months if stored at 4 °C. Do not freeze.
For some cell types, a gelling procedure using 7.5% (w/v) Sodium Bicarbonate for neutralization may be preferred.
Mammary epithelial cells, MCF-10A cultured on 3-D Culture Matrix™ Collagen I are enduced to differentiate with the addition of 3-D Culture Matrix™ Laminin-1 at: a) 0 mg/mL, b) 1 mg/mL, and c) 2 mg/mL.
3-D Culture Matrix is a trademark of Trevigen, Inc.
Cultrex is a registered trademark of Trevigen, Inc.
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