A. Cryopreserved Vials (Cn354-05)
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liquid nitrogen in the cryovials to burst and cause injury.
Canine Aortic Smooth Muscle Cells (CnAOSMC)
A. Preparing Cell Culture Flasks for Culturing CnAOSMC
B. Thawing and Plating CnAOSMC
A. Preparing Subculture Reagents
B. Preparing Culture Flask
C. Subculturing CnAOSMC
Trypsinize Cells at Room Temperature. Do Not Warm Any Reagents to 37 °C.
A. Seeding CnAOSMC
B. Inducing CnAOSMC