A. Cryopreserved Vials (352-05a)
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liquid nitrogen in the cryovials to burst and cause injury.
Figure 1.Human Pulmonary Artery Smooth Muscle Cells (HPASMC)
A. Preparing Cell Culture Flasks for Culturing HPASMC
B. Thawing and Plating HPASMC
A. Preparing Subculture Reagents
B. Preparing Culture Flask
C. Subculturing HPASMC
Trypsinize Cells at Room Temperature. Do Not Warm Any Reagents to 37 °C.
A. Seeding HPASMC for Differentiation
B. Differentiating HPASMC To Express Contractile Protein
Research. Development. Production.
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