After thawing and plating the cryopreserved cells, the first medium change should be done after 24 hours or overnight, so that both residual DMSO and any dead cells are removed. Thereafter, the medium should be changed every 48 hours until the cells are ready to be passaged. More specific instructions are included in each cell line’s protocol.
For this purpose, the term "population doubling" is used instead of "passage" for describing the growth potential of the cells. A population doubling is a two-fold increase in the total number of cells in culture. A passage is the propagation of a cell population by subculturing from one vessel to 3 or 4 vessels. Because different researchers use different split ratios when they subculture, it is difficult to predict how many passages can be obtained with a particular primary cell. Our primary cells are guaranteed for a specified number of population doublings (as indicated within the product descriptions) when the corresponding cell-specific growth media are used for the culturing procedures.
The following equations can be used to determine the maximum number of culture vessels that can be set up:
The effective growth areas for common tissue culture ware are listed below:
No, our complete growth media are fully supplemented and ready-to-use for your convenience. Each complete medium contains all the essential components for optimized cell growth.
Yes, we offer the basal media and growth supplements packaged individually.
For optimal cell growth please follow the handling instructions below.
Most cells we offer can be plated on standard tissue culture grade ware. However, some cell types require coating material. For specific instructions, please see the respective cell product web pages to access the subculturing protocols.
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