1 base (Cs2CO3, ground and sieved)
2 solvents (1,4 Dioxane and DMAc)
12 pre-weighed catalysts
Additional (not included) items needed:
Solutions & slurries to make: Go to the online user set-up page to enter molecular weights into the downloadable excel file.
-49 mL CH3CN
-1 mL AcOH
-15.4 mg Biphenyl (KitAlysis Internal Standard provided in the kit)
Note: This recipe makes 50mL which is enough stock solution for all four screening set in the KitAlysis Buchwald-Hartwig Amination Reaction Screening Kit
Sealing the Plate-screwing down the cover
Sealing the plate properly is critical to success. The key is light even pressure to the lid of the block to keep the cover flat while sealing.
Use a small oil bath or other metal block (for best results) in the back of the hot plate and place the probe in there. Place reaction block as close to the center of the hot plate as possible for more even stirring.
Due to narrow opening of pipette tips, slurry additions will result in blockages. To aid in uniform dosing, simply snip off the end of the tip at the first marker (~10 mm). To ensure an evenly dispersed aliquot, it is critical that the mixture is stirring while you draw aliquots for dosing into the corresponding reaction vial.