Video: PCR Clean-Up Procedure

Product No. NA1020

The GenElute™ PCR Clean-up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. DNA purification is achieved in a few easy steps. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

  • Starting Amount - 100 µL or 10 µg of DNA
  • Yield - Recovers up to 95% of PCR fragments between 100 bp to 10 kb
  • Speed - Purify up to 100 µL or 10 µg of PCR amplified DNA in 5 minutes
  • Flexible - DNA concentrated in 50 µL of buffer
  • Economic - 40% more purifications than market leader

Sufficient for 70 purifications.

PCR Clean-Up Steps

  1. Insert the binding column into a provided collection tube.

  2. Add 0.5 mL of the column preparation solution to each miniprep column.

  3. Centrifuge at 12,000 x g for 1 minute.

  4. After 1 minute, discard the eluate.

  5. Transfer PCR reaction mix into each tube.

  6. Add 5 volumes of binding solution to 1 volume of the PCR reaction and mix.

  7. Transfer the solution into the binding column.

  8. Centrifuge at maximum speed for 1 minute.

  9. After 1 minute, discard the eluate, but retain the collection tube.

  10. Add 0.5 mL of diluted wash solution to the column.

  11. Centrifuge at maximum speed for 1 minute.

  12. After 1 minute, discard the eluate, replace the column back into the collection tube.

  13. Centrifuge at maximum speed for 2 minutes (without adding additional wash solution.

  14. After 2 minutes, discard any residual eluate and the collection tube. Transfer the column to a fresh 2 mL collection tube.

  15. Add 50 µL of elution solution to the center of each column.

  16. Centrifuge at maximum speed for 1 minute.

  17. After 1 minute, discard the column.

  18. PCR amplification products are now presented in the eluate.

Materials
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