Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
- If your total RNA sample quantity is less than 1 microgram, amplify the experimental and control (input) samples using the Transplex WTA Kit (WTA1) or the Complete WTA2 Kit (WTA2-50RXN) before labeling.
- The WTA amplification product ranges from ~200 to 1000 base pairs in size, averaging ~300 base pairs, making fragmentation unnecessary. This size is sufficiently large for NimbleGen microarray target.
- Purify samples using the GenElute™ PCR Cleanup kit (NA1020).