The video above follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

  • Cells or tissue deposited on slides or in microplates are fixed to preserve activation status and transient interactions.
  • Validated primary antibodies for the targets are added followed by binding of the PLA probes.
  • Hybridization of the oligonucleotide arms of the PLA probes will create a template for rolling circle amplification (RCA) only when the epitopes of the target proteins are in close proximity (<40 nm)
  • Amplification and labeling of the RCA product by detection probes.
  • The resulting spot can be detected and visualized by standard microscopy.
  • Images can easily be quantitatively analyzed using the Duolink Image Tool software, which facilitates both average and single cell data analysis.

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.