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Tyrosine kinases (PTK) represent only 10% of all protein kinases, although they are the most important protein kinases. PTKs are involved in cell growth signaling. Protein tyrosine phosphatases (PTP) attenuate growth signals generated by the PTKs through catalyzing the tyrosine dephosphorylation step on their substrate proteins. PTPs also are fundamental in cell cycle regulation and activation. Knowledge of the overall expression pattern of PTKs and PTPs in any given cancer cell represents the first step in understanding the sequential events of tumor progression.
The following functional assay is an excerpt from the book "Cancer Cell Signaling: Methods and Protocols" © 2003 Humana Press. This method is one of several detailed assays featured in this book. The book also contains detailed protocols on the manipulation and detection of signals and protein interactions.
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
From Methods in Molecular Biology, Vol. 218, Cancer Cell Signaling: Methods and Protocols pp. 113-125
David M. Terrian, Editor
Humana Press Inc., Totowa NJ, U.S.A.
© 2003 Humana Press
Clarification: The term "we" in this protocol refers to Dr. Lin and his associates.