Coronavirus qPCR & Related Products to Support SARS-CoV-2 Research

2019-nCoV Assays for Detection of SARS-CoV-2

US-2019-nCoV and EUR-2019-nCoV Kits and Individual Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2. These products are primer and probe pools, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (US-2019-nCoV) and Charité Institute of Virology – Universitätsmedizin Berlin (EUR-2019-nCoV).

All products have been extensively tested and found to perform well with Thermo Fisher Scientific’s TaqPath™ 1-Step RT-qPCR Master Mix and our own Quantitative RT-PCR ReadyMix (QR0200) (see figure for example data).

Example US-2019-nCoV data: N2 Individual Assay compared against that of a competing product. The N2 individual assay was functionally validated against ATCC SARS-CoV-2 reference material and found to be template free up to 45 cycles. The assay is sensitive with a limit of detection of 1 to 10 copies. Comparison was completed with QR0200 ReadyMix with the reverse transcription at 44 °C for 30 min. A) Competing assay B) US-2019-nCoV N2 Individual Assay lot 1 C) US-2019-nCoV N2 Individual Assay lot 2 D) Competing product and US-2019-nCoV N2 Individual Assay amplification plots. NTC = no template control and PC = positive control.

Features & Benefits

  • Convenient 20X formulation
  • 1000 reactions per each assay
  • Functional testing of each lot
  • In inventory for rapid delivery

Order Sigma-Aldrich-Designed SARS-CoV-2 Primers & Probes

Predesigned Primers & Probes

Our qPCR assay designs are available for immediate ordering from our KiCqStart® product lines.

Our KiCqStart® Dual-Labeled Probes are labeled with our own license and royalty-free dark quencher, Onyx Quencher (OQ). OQ is also available with custom probe orders. To learn more about OQ beyond what is on the landing page, please contact us.

For Research Use Only. Not for Use In Diagnostic Procedures.

Custom Primers & Probes

We offer probes labeled with either Onyx Quencher (OQ) or Block All Quencher (BXQ). To request a quote for custom primers and probes, please email us.

Single-Stranded RNA Positive Controls

Custom

The SARS-CoV-2 virus has a positive-sense ssRNA genome. We offer in vitro transcribed ssRNA against regions of this genome, which can serve as positive controls. We can synthesize custom sequences from 150 to 420 nucleotides (to ensure successful manufacturing, please avoid sequences with inverted repeats and repetitions). For Research Use Only. Not for Use In Diagnostic Procedures.

To request a quote or additional help, please email us.

Predesigned

Single stranded RNA (ssRNA) Reference Materials intended for the use as a positive quality control sample for coronavirus SARS-CoV-2 in real-time RT-PCR assays.

For Research Use Only. Not for use in diagnostic procedures.

RT-PCR and RT-qPCR Genetic Analysis Reagents

We are committed to providing researchers with the reagents and technical expertise for timely support of the SARS-CoV-2 outbreak. Explore our offering of world-class RT-PCR, RT-qPCR and supporting reagents that are suitable for SARS-CoV-2 vaccine research.

Transcriptor One-Step RT-PCR Kit

Utilizing hot start one-step RT-PCR technology, Transcriptor One-Step RT-PCR Kit includes a mixture of enzymes, including Taq DNA polymerase and a proofreading polymerase, to ensure sensitive and robust reverse transcription. Except for template and primers, Transcriptor One-Step RT-PCR Kit provides all the components required for one-step RT-PCR.

KAPA PROBE FAST One-Step

KAPA PROBE FAST One-Step is designed for high-throughput, fast-cycling, one-step RNA quantification. KAPA PROBE FAST One-Step is suitable for use with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).

Titan™ One Tube RT-PCR System

Incorporating a mixture of three enzymes, Titan™ One Tube RT-PCR System utilizes reverse transcriptase AMV for first-strand cDNA synthesis and the Expand™ High Fidelity enzyme blend, consisting of Taq DNA polymerase and a polymerase with a proofreading activity, for amplification of cDNA by PCR.

Quantitative RT-PCR ReadyMix™

In addition to containing Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) for first-strand cDNA synthesis, the Quantitative RT-PCR ReadyMix™ is conveniently blended with JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, stabilizers, and ships as a 2x concentrate.

KiCqStart® One-Step Probe RT-qPCR ReadyMix™

Suitable for all dual-labeled probe chemistries, KiCqStart® One-Step Probe RT-qPCR ReadyMix™ comes with all required components for RT-qPCR, except for RNA template and probe. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is available with or without ROX™ reference dye.

SYBR® Green Quantitative RT-qPCR

Providing a highly sensitive method for the quantitative analysis of gene expression, this kit combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix™.

RT-PCR, RT-qPCR and Supporting Reagents
Loading

For Research Use Only. Not for Use In Diagnostic Procedures.

Proficiency Testing

In support of quality assurance efforts, the SARS-CoV-2 proficiency testing kit is designed for use with assays like real-time RT-PCR which detect the viral RNA by nucleic acid amplification. Reports provide evaluation of qualitative results as well as quantitative properties such as Cq(Ct) values and efficiencies.

RNA Interference

Our MISSION® esiRNA COVID-19 panel (51 genes with 2 controls) provides RNAi researchers with a proven, cost-effective, and simple way to perform post-transcriptional silencing of putative genes involved in the intracellular life cycle of SARS-CoV-2. Included in the panel are genes that were found to be involved with the infection caused by other coronaviruses, such as SARS-CoV and MERS. This panel is available as a customizable esiFLEX 96-well array at quantities up to 5.0 μg, which is sufficient for screening common human cell lines.

Human Genes in COVID-19 esiRNA Panel
Loading

Included with the panels is the following:

  • Positive control: Human Eg5 (KIF11) to ensure transfection & experimental set up optimization
  • Negative control: RLUC to distinguish sequence-specific silencing from non–specific effects

To request a quote or additional help, please email us.

For Research Use Only. Not for Use In Diagnostic Procedures.

Please pass along our biochemicals for Vaccine and Diagnostics research (Download PDF). Contact us for expedited support in SARS-CoV-2 vaccine development.

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.