Water Solutions: Solubilizing some lipids is difficult using organic solvents. Typically, lipids are suspended in methanol to aliquot, the methanol is then dried and the residue dispersed using fatty acid-free BSA solution. A general procedure for the preparation of a lipid stock solution is as follows:
Stock Solution: 125 µM of lipid in essentially fatty acid-free bovine serum albumin (BSA, 4 mg/mL)
Preparation of Stock Solution: Methanol Solution:
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