Domestic wastewater and sewage monitoring are essential for protecting public health and ensuring clean water in the environment. Through the Clean Water Act, the Environmental Protection Agency (EPA) and individual municipalities are responsible for directly governing wastewater testing strategies and procedures. The EPA both issues and approves testing methods for a wide variety of contaminants and analytes found in wastewater including trace metals, nonmetals, salts, organic compounds, bacteria, viruses, and particles such as asbestos or silica. Individual municipalities dictate what tests are necessary, how often these tests are conducted, and how data are organized.

Wastewater treatment site

Many of the EPA’s sanctioned testing methods involve filtration for sample preparation or processing. Because a variety of filter types and formats can be successfully employed for many of these methods, the method may neglect to specify which filters are best suited beyond defining one or two parameters. For example, ASTM D3867-16 (Standard Test Methods for Nitrate-Nitrate in Water) directs the scientist to “purchase suitable filter paper… material such as fine-textured, acid-washed ashless paper or glass fiber paper are acceptable”.

This tech article will help serve as a guide to determine which filters can most successfully be applied in these methods to assist lab and processing plants in selecting the best products for their needs.

Clarification and Pre-filtration

When handling and processing wastewater and sewage, pre-filtration is very often a necessary stage in sample preparation to reduce fowling and improve the efficacy of downstream microfiltration. To designate pre-filtration, methods will sometimes call for “filter paper”, “coarse filter paper”, or simply indicate “filtration” in an early clarifying or particle-removing step. Pre-filtration requires an open mesh or net filter and can best be accomplished using options such glass fiber filters or quartz fiber filters for aqueous samples, or polypropylene filters for solvent filtration. Both pre-filtration choices have broad thermal compatibility and high retentate capacities for viscous and high particulate samples common in wastewater testing.

General Filtration of Wastewater Samples

Many EPA and EPA-recommended methods do not specify filter type. They may designate parameters or specifications such as a pore size or format (disc or syringe filter) without detailing the filter material. Hydrophilic polyvinylidene fluoride (PVDF), polytetrafluoroethylene (PTFE), mixed cellulose esters (MCE), and cellulose acetate filters are all valid options for general filtration. Millipore® Durapore® PVDF and Omnipore™ PTFE filters are ideal for low-protein binding applications. Millex®-HA MCE syringe filters and MF-Millipore® MCE disc membranes are biologically inert, low binding, and thermally stable with a high loading capacity. All three membrane filters provide low extractable filtration for minimal contamination. LCR PTFE filters are specially treated to prevent the introduction of additional extractables to your sample prior to HPLC analysis or similar instrumentation.

Scanning electron micrographs of PVDF, PTFE, and MCE membrane filters

Scanning electron micrographs (SEM) of membrane filters. A) PVDF membrane, B) PTFE membrane, C) MCE membrane.

Filter Size and Format

One of the primary considerations when selecting a filter format is sample size and volume. When filtering small volumes < 100 mL, a syringe filter is often the best choice. Because of hold up or “dead” volume considerations, the smallest possible diameter of filter should always be used. Generally, 4 mm diameter filters should be used for volumes < 1 mL, 13 mm diameter filters for volumes in the 1-10 mL range, and 25 mm or 33 mm diameter filters for volumes between 10 mL - 100 mL.

For volumes exceeding 100 mL, a vacuum filtration setup with cut disc filters is generally preferred. Consider a 47 mm filter for volumes ranging between 120 mL and 1 L. For sample volumes over a liter, a 90 mm cut disc can help facilitate a higher throughput and suitable flowrate.

Syringe filters and cut disc membrane filters
Table 1.Recommended filter size by sample volume.

Hardware and Filtration Accessories

Varying filter formats require different filtration setups and workflow optimization tools. Sample composition and throughput can influence which setup will be most appropriate in terms of both convenience and practical considerations.

Cut disc formats necessitate vacuum filter holders and accessories. Glass setups can be used for aqueous solutions as well as solvents or corrosive liquids (except when specifically prohibited) and come in 13 mm, 25 mm, 47 mm, and 90 mm sizes/diameters. For non-sterile buffer filtration, conventional glass vacuum systems or the Millicup™-FLEX disposable vacuum filtration unit can also be used with any suitable cut disc membrane. The Millicup™-FLEX Disposable Filtration Unit offers a convenient, recyclable, disposable alternative to traditional glass setups that is suitable for a wide range of sample volumes. The Millicup™-FLEX filtration unit offers a safe and efficient means of filtering a variety of aqueous solutions or solvents directly into GL45 vacuum-rated filtration bottles using 47 mm disc membranes. Both glass and Millicup™-FLEX vacuum filtration options can accommodate cut disc membranes of all material types.

When using Millex® Syringe Filters to filter multiple samples, especially those that are viscous or particle laden, the vacuum-driven Samplicity® G2 Filtration System can offer an ergonomic solution to hand fatigue and bulk manual processing. The Samplicity® G2 Filtration System can quickly vacuum filter up to eight samples at a time directly into HPLC vials, improving efficiency for pre-instrument preparation. In conjunction with, or as an alternative to, the Samplicity® G2 Filtration System, Millex® HPF filters incorporate a graduated glass fiber pre-filter (0.7 - 10.0 µm) to remove larger particles prior to microfiltration. This can further assist in processing difficult samples.


Recommended Filtration Products by Method

Sample volume:

  • 2-3 mL

Filter specifications:

  • 0.2 µm or 0.45 µm membrane pore size

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Sample volume:

  • 10 – 25 mL

Filter specifications:

  • 0.2 µm pore size
  • 10- to 25-mL filter-adaptable HDPE, polypropylene or glass syringes with luer lock adapters

Recommended filtration products:

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Filter specifications:

  • 0.2 µm or 0.45 µm membrane pore size
  • Cellulose Acetate; Nylon

Recommended filtration products:

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Filter specifications:

  • Borosilicate glass

Recommended filtration products:

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Filter specifications:

  • 0.2 µm or 0.45 µm cut disc membrane

Recommended filtration products:

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Sample volume:

  • 500 mL, 100 mL test sample

Filter specifications:

  • 0.45 µm filter paper

Recommended filtration products:

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Sample volume:

  • 50 mL

Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Sample volume:

  • 1 L

Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Sample volume:

  • 100 - 150 mL

Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Sample volume:

  • 100 - 200 mL

Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size
  • Use a glass fiber filter without organic binder or else filters made of TFE, polycarbonate, silver, polyethersulfone, or hydrophilic polypropylene. Other filters that neither adsorb nor leach organic carbon into water may be used, especially if colloidal matter must be removed.

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size
  • Preferably HPLC syringe filters or similar, with no detectable DOX blank. Rinsed glass-fiber filters are satisfactory.

Recommended filtration products:

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Filter specifications:

  • 0.45 µm silver membrane
  • All glass filtering device

Recommended filtration products:

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Sample volume:

  • 10 mL

Filter specifications:

  • 0.45 µm PTFE membrane, 13 mm syringe filter

Recommended filtration products:

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Sample volume:

  • 10 mL, 500 -1 L format

Filter specifications:

  • 0.2 µm or 0.45 µm membrane pore size

Recommended filtration products:

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Sample volume:

  • 100 mL

Filter specifications:

  • 0.45 µm membrane pore size
  • Fine textured, acid washed, ashless paper or glass fiber paper

Recommended filtration products:

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Sample volume:

  • 250 mL

Filter specifications:

  • 0.45 µm membrane pore size
  • Fine textured, acid washed, ashless paper or glass fiber paper

Recommended filtration products:

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Filter specifications:

  • 0.45 µm membrane pore size

Recommended filtration products:

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