Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPLC and UHPLC instruments. The column efficiency performs typically 40% higher in comparison to fully porous particulate columns with the same particle size.
The Ascentis® Express C18 bonded phase is nonpolar in nature. It is best used with mobile phases that are mixtures of methanol and water or acetonitrile and water. Higher levels of the organic solvent component will typically reduce the retention of the sample compounds. Using elevated temperatures (e.g., 40–60 ºC) will reduce the viscosity of the mobile phase and allow the use of faster flow rates and lower column pressure for high sample throughput.
Ascentis® Express C18 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:
2.0 µm particles
Best Fused-Core UHPLC Column
An optimized solution for high-throughput small molecule analysis
2.7 µm particles
Fast HPLC on ANY System
A practical solution that delivers UHPLC performance from any HPLC
5.0 µm particles
The Lab Work-Horse Column
True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns
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