LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18
Cory Muraco
MilliporeSigma, Bellefonte, PA
Introduction
An Ascentis® Express C18 column was used for the LC-MS/MS analysis of bile acids and their conjugates. The separation of multiple isobaric compounds was demonstrated in addition to excellent linearity, enabling clinically relevant concentrations to be analyzed.
Experimental Conditions for Analysis of Bile Acids |
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Performance results for the Separation of Bile Acids on Ascentis® Express C18
Figure 1.LC-MS Results for the Separation of Bile Acids on Ascentis® Express C18. Numbering corresponds to elution order as indicated on the right.
Peak Identities
- Sodium tauroursodeoxycholate (TUDC)
- Glycoursodeoxycholic acid (GDC)
- Taurocholic acid sodium salt hydrate (TCA)
- Glycocholic acid hydrate (GCA)
- Sodium taurochenodeoxycholate (TCDC)
- Ursodeoxycholic acid (UDC)
- Sodium taurodeoxycholate hydrate (TDC)
- Sodium glycochenodeoxycholate (GCDC)
- Cholic acid (CA)
- Sodium taurolithocholate (TLC)
- Chenodeoxycholic acid (CDC)
- Deoxycholic acid (DC)
- Lithocholic acid (LC)[MN1]
Linearity of Assay for Each Compound
Linearity of Assay for the bile acids and conjugates used in their LC-MS analysis
Figure 2.Sodium tauroursodeoxycholate (TUDC)
Figure 3.Taurocholic acid sodium salt hydrate (TCA)
Figure 4.Glycoursodeoxycholic acid (GDC)
Figure 5.Glycocholic acid hydrate (GCA)
Figure 6.Sodium taurochenodeoxycholate (TCDC)
Figure 7.Sodium taurodeoxycholate hydrate (TDC)
Figure 8.Ursodeoxycholic acid (UDC)
Figure 9.Sodium glycochenodeoxycholate (GCDC)
Figure 10.Cholic acid (CA)
Figure 11.Chenodeoxycholic acid (CDC)
Figure 12.Sodium taurolithocholate (TLC)
Figure 13.Deoxycholic acid (DC)
Figure 14.Lithocholic Acid (LC)
Conclusion
Excellent resolution and linearity were obtained for the isobaric bile acids on the Ascentis® Express C18 column.