Skip to Content
MilliporeSigma
HomeSmall Molecule HPLCmanu-CART® cartridge holder for LiChroCART® HPLC cartridge

manu-CART® cartridge holder for LiChroCART® HPLC cartridge

Introduction

The versatile and reusable manu-CART® cartridge holders are designed to fit a range of LiChroCART® cartridge lengths and internal diameters. Since these cartridge holders may remain in the system, and the capillary connections do not need to be detached, the cartridges may be changed within the shortest possible time. This flexibility broadens the scope of HPLC applications, allowing for seamless cartridge integration with merely a twist of the hand.

The LiChroCART® cartridge column hardware is available for Purospher®, Purospher® STAR, LiChrospher®, Superspher®, ChiraDex® and LiChrosorb® HPLC columns and customized products on demand. 

On the left, there is a fully assembled piece consisting of a cap nut with a knurled surface, designed for easy gripping and fastening. It connects to another metallic section, forming a cylindrical shape. On the right, the parts of the cartridge holder are disassembled: two cap nuts are displayed alongside four smaller cylindrical components (spin collets), and two larger threaded end fittings.

manu-CART® NT cartridge holder

The re-usable manu-CART® NT cartridge holder seamlessly accommodates cartridges with diameters of 2, 3, 4, and 4.6 mm, along with lengths of 75, 100, 125, 150 and 250 mm. A simple turn permits an easy and problem-free integration of a LiChroCART® 4-4 guard cartridge.

The manu-CART® NT cartridge holder consists of two cap nuts, four spin collets, and two endfittings.

Mounting manu-CART® NT holder to a LiChroCART® cartridge without a guard-column

These instructions describe how to fit the pieces to just one end of your cartridge. Repeat steps 1 to 4 for the other end.

 An assembly process where two components are being connected. The top part is a cylindrical piece that attaches to a lower component, which is a threaded fitting connected to tubing. Two half-circle clips are inserted from the sides into a groove on the threaded fitting to ensure a secure connection between the parts. An arrow indicates the top part should be attached by pushing it downward onto the threaded fitting, allowing for a firm, reliable connection for fluid transfer. This assembly does not require an additional precolumn.
  1. Insert the cartridge through the endfitting, so that the outer thread is at the outermost end.
  2. Place two split collets around the neck of the cartridge as shown in the figure on the left.
  3. Push the endfitting up over the 2 split collets.
  4. Screw the end cap over the assembled endfitting. Tighten it finger-tight.
  5. When you install your column into the flow path of your LC, tighten the endfittings leak-tight using a wrench.

Mounting manu-CART® holder to a LiChroCART® cartridge with integration of a LiChroCART® 4-4 guard cartridge

These instructions describe how to fit the pieces to just one end of your cartridge.

An assembly process where two components are connected, with an additional intermediary part being inserted between them. The top piece is a cylindrical object being directed downward to fit onto the lower threaded piece connected to tubing. In the middle, a smaller cylindrical insert is shown being placed into the fitting before the two main components are joined. On the sides, two half-circle clips are being inserted into grooves on the threaded piece to secure the connection. This setup shows how to assemble the system when an extra component is involved, ensuring a secure and reliable connection for fluid transfer.
  1. Insert the cartridge through the endfitting, so that the outer thread is at the outermost end.
  2. Place two split collets around the neck of the cartridge as shown in the figure on the left.
  3. Place the guard column cartridge inside the space at the end of the collets.
  4. Push the endfitting up over the assembled guard column.
  5. Screw the end cap over the assembled endfitting. Tighten it finger-tight.
  6. When you install your column into the flow path of your LC, tighten the endfittings leak-tight using a wrench.

Mounting Shorter LiChroCART® HPLC cartridges

Short LiChroCART® cartridges with 30 mm and 55 mm lengths require a separate manu-CART® holder.

A metallic assembly of manu-CART® holder 30 mm for LiChroCART 30-4, 30-3, and 30-2 cartridges. On the left is a partially assembled holder with a threaded end and a knurled surface for gripping. It has a black connector or stopper protruding from one end, for securing tubing or similar components. In the center, there is a separate cylindrical metal piece, likely an internal cartridge or spacer. On the right, another knurled cap is displayed, similar in design to the first, with a black stopper at its end.

manu-CART® holder 30 mm (1.50227) for LiChroCART® 30-4, 30-3 and 30-2

A metallic assembly of manu-CART® holder 55 mm for LiChroCART® 55-4, 55-3, and 55-2 HPLC cartridges. The components are laid out in an exploded view. On the left is a knurled cylindrical holder with a black stopper or connector at one end and a threaded portion at the opposite end, which allows it to be screwed into other components. In the center, a smooth metal cylindrical tube is positioned, likely representing the internal cartridge or spacer. On the right is another smaller knurled cap that also features a black stopper at its end.

manu-CART® holder 55 mm (1.50226) for LiChroCART® 55-4, 55-3 and 55-2

These cartridge holders have one tube (30 or 55 mm in length) and two endfittings that suit columns with internal diameters of 2 mm, 3 mm, and 4 mm. To assemble, slide the tube onto the cartridge and secure the end fittings onto both ends of the tube.

 

manu-CART® coupling unit to connect two LiChroCART® cartridges and additional manu-CART® holders

Additionally, a coupling unit facilitates the seamless connection of two LiChroCART® cartridges, with an optional kit provided for linking a LiChroCART® cartridge to a LiChroCART® 25×4 pre-cartridge.

LiChroCART® cartridges with 10 mm I.D. require the re-usable manu-CART® “10” II holder which can be used in the same way as the manu-CART® NT holder with or without integration of a corresponding LiChroCART® 10-10 guard-cartridge.

 

manu-CART® cartridge holders for stainless steel LiChroCART® cartridges

Description

Cat. No.

Contents of one package

manu-CART® NT cartridge holder for 2, 3, 4, and 4.6 mm I.D. LiChroCART® cartridges

1.51486.0001
2 complete stainless-steel units for mounting one LiChroCART® cartridge

manu-CART® “10” II cartridge holder for 10 mm I.D. LiChroCART® cartridges

1.51419.0001

2 complete stainless-steel units for mounting one LiChroCART® 10 mm I.D. cartridge

manu-CART® coupling kit for coupling with LiChroCART® 25-4 pre-cartridge

1.50082.0001

1 coupling unit 1 endfitting for LiChroCART® 25-4

manu-CART® coupling unit to connect two LiChroCART® cartridges

1.50083.0001 

1 piece

manu-CART® holder 25-4 and 25-2

1.50017.0001 

1 piece

manu-CART® holder 30 mm for 30-2, 30-3 and 30-4 LiChroCART® cartridges

1.50227.0001 

1 piece

manu-CART® holder 55 mm for 55-2, 55-3 and 55-4 LiChroCART® cartridges

1.50226.0001 

1 piece
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?