MilliporeSigma
HomeSmall Molecule HPLCUHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor

UHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor

William L. Maule III, Clint Corman, Michael Ye, Cory Muraco, Curtis Frantz

MilliporeSigma Bellefonte, PA

Introduction

Nucleosides are an important class of small molecules that are the building blocks of nucleic acids. In addition, nucleosides have found interest as active pharmaceutical ingredients (APIs) in antiretroviral drugs and as biomarkers for certain diseases. However, due to the structural similarity between these analytes, developing an analytical method to characterize a series of these compounds can be challenging. This application demonstrates the use of the SupelTM Carbon LC column to resolve a set of 12 nucleosides.

2-D chemical structures (Bond line structure) of nucleosides used in the study to resolve 12 nucleosides by Ultra high performance liquid chromatography (UHPLC)

Figure 1.Chemical Structures of Compounds Used in this Study.

Experimental conditions for Nucleoside analysis

Table 1.Chromatographic Conditions for Analysis of Nucleosides.
Table 2.Components of Test Mix Used in Study.

Performance results and comparison

Chromatograms showing peaks obtained for the analysis of Twelve Nucleosides on Supel™ Carbon LC

Figure 3.Analysis of Twelve Nucleosides on Supel™ Carbon LC.

Table 3.Elution Order for Nucleosides on Supel™ Carbon LC.
Chromatograms showing peaks obtained for the analysis of Twelve Nucleosides on Competitor Column

Figure 4.Analysis of Twelve Nucleosides on a Competing Carbon Column.

Table 4.Elution Order for Nucleosides on Competing Carbon Column.

Conclusion

This application has demonstrated the use of SupelTM Carbon LC column in resolving 12 nucleosides in under 15 minutes. The SupelTM Carbon LC column also outperforms a competitor column where two co-elutions occur and is an excellent choice for use in nucleoside biomarker identification and quantitation.

Materials
Loading
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?