HyStem™ is a semi-synthetic 3D hydrogel platform that allows cell growth in a 3D environment. Their main component is one of the major constituents of the extracellular matrix (ECM), hyaluronic acid (HA). HA is the simplest glycosaminoglycan (a negatively charged, linear, non-sulfated polysaccharide) that provides compression strength, lubrication, and hydration within the ECM1. It also regulates cell adhesion and motility2,3 and mediates cell proliferation and differentiation4, making it not only a structural component of tissues but also an active signaling molecule. Hystem™ hydrogels have been extensively published for numerous applications including stem cell, cancer and neuroscience research.
Three different HyStem™ platform options are available:
Figure 1. Biochemical structure of Hystem™ hyaluronic acid based 3D hydrogels.HyStem™ HA hydrogels closely mimic the native extracellular matrix protein environment giving customers options of HA, HA+ collagen or HA+ collagen+ heparin sulfate linked hydrogels for 3D cell culture support and growth. The hydrogels are customizable and allow attachment of other growth factors and ECM proteins.
A hyaluronan hydrogel scaffold-based xeno-free culture system for ex vivo expansion of human corneal epithelial stem cells. Chen D, Qu Y, Hua X, Zhang L, Liu Z, Pflugfelder S C and Li DQ. Eye (Lond). 2017 Jun;31(6):962-971. Demonstrates that HyStem™ C can successfully support the ex vivo cultivation of human corneal epithelial stem cells (CESCs). It has been shown several times that HyStem™ C is a very suitable carrier for ex vivo experimentation with numerous types of stem cells.
Mesenchymal stem cells support growth and organization of host-liver colorectal-tumor organoids and possibly resistance to chemotherapy. Devarasetty M, Wang E, Soker S, Skardal A. Biofabrication. 2017 Jun 7;9(2):021002. This paper used HyStem™ HP in a study of host-liver colorectal-tumor spheroids composed of primary human hepatocytes, mesenchymal stem cells (MSC) and colon carcinoma HCT116 cells were created in simulated microgravity rotating wall vessel (RWV) bioreactors. The cells were seeded on HyStem™ microcarriers, loaded with liver-specific growth factors and ECM components.
3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro. Mannino RG, Santiago-Miranda AN, Pradhan P, Qiu Y, Mejias JC, Neelapu SS, Roy K, Lam WA. Lab Chip. 2017 Jan 31;17(3):407-414. UsedHyStem™ C to develop a lymphoma-on-chip model consisting of a hydrogel based tumor model traversed by a vascularized, perfusable, round microchannel that successfully recapitulates key complexities and interactions of the in vivo tumor microenvironment in vitro.
Cell-cell interaction between vocal fold fibroblasts and bone marrow mesenchymal stromal cells in three-dimensional hyaluronan hydrogel. Chen X, Thibeault SL. J Tissue Eng Regen Med. 2016 May;10(5):437-46. This paper used HyStem™ C for coculture of vocal fold fibroblasts (VVF) and bone-marrow deriver mesenchymal stromal cells (BM-MSCs).
Multilayered Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures. Engel BJ, Constantinou PE, Sablatura LK, Doty NJ, Carson DD, Farach-Carson MC, Harrington DA, Zarembinski TI. Adv Healthc Mater. 2015 Aug 5;4(11):1664-74. This paper used HyStem™ C to develop HTS suitable for 3D co-culture systems, mixing cancer and stromal cells.