Basal Media Formulation

Component	B1522 [1x] g/L	B9638 g/L
Inorganic Salts
Calcium Chloride	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	2.2	—
Sodium Chloride	6.8	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122
Amino Acids
L-Arginine • HCl	0.021	0.021
L-Cystine • 2HCl	0.01565	0.01565
L-Glutamine	—	0.292
L-Histidine (free base)	0.008	0.008
L-Isoleucine	0.026	0.026
L-Leucine	0.026	0.026
L-Lysine • HCl	0.03647	0.03647
L-Methionine	0.0075	0.0075
L-Phenylalanine	0.0165	0.0165
L-Threonine	0.024	0.024
L-Tryptophan	0.004	0.004
L-Tyrosine • 2Na • 2H₂O	0.02595	0.02595
L-Valine	0.0235	0.0235
Vitamins
D-Biotin	0.001	0.001
Choline Chloride	0.001	0.001
Folic Acid	0.001	0.001
myo-Inositol	0.002	0.002
Niacinamide	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001
Pyridoxal • HCl	0.001	0.001
Riboflavin	0.0001	0.0001
Thiamine • HCl	0.001	0.001
Other
D-Glucose	1	1
Phenol Red • Na	0.011	0.011
Add
L-Glutamine	0.292	—
Sodium Bicarbonate	—	2.2

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