Home Mammalian Cell CultureMcCoy's 5A Modified Media Formulation

McCoy's 5A Modified Media Formulation

Component	M4892 g/L	M8403 [1x] g/L
Inorganic Salts
Calcium Chloride	0.09995	0.09995
Magnesium Sulfate (anhydrous)	0.0976876	0.0976876
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	—	2.2
Sodium Chloride	6.46	6.46
Sodium Phosphate Monobasic (anhydrous)	0.504	0.504
Amino Acids
L-Alanine	0.01336	0.01336
L-Arginine • HCl	0.04214	0.04214
L-Asparagine • H₂O	0.04503	0.04503
L-Aspartic Acid	0.01997	0.01997
L-Cysteine	0.02424	0.02424
L-Glutamic Acid	0.02207	0.02207
L-Glutamine	0.21915	—
Glycine	0.00751	0.00751
L-Histidine • HCl • H₂O	0.02096	0.02096
Hydroxy-l-proline	0.01967	0.01967
L-Isoleucine	0.03936	0.03936
L-Leucine	0.03936	0.03936
L-Lysine • HCl	0.03654	0.03654
L-Methionine	0.01492	0.01492
L-Phenylalanine	0.01652	0.01652
L-Proline	0.01727	0.01727
L-Serine	0.02628	0.02628
L-Threonine	0.01787	0.01787
L-Tryptophan	0.00306	0.00306
L-Tyrosine • 2Na • 2H₂O	0.0261	0.0261
L-Valine	0.01757	0.01757
Vitamins
Ascorbic Acid	0.0005625	0.0005625
p-Aminobenzoic Acid	0.001	0.001
D-Biotin	0.0002	0.0002
Choline Chloride	0.005	0.005
Folic Acid	0.01	0.01
myo-Inositol	0.036	0.036
Niacinamide	0.0005	0.0005
Nicotinic Acid	0.0005	0.0005
D-Pantothenic Acid (hemicalcium)	0.0002	0.0002
Pyridoxal • HCl	0.0005	0.0005
Pyridoxine • HCl	0.0005	0.0005
Riboflavin	0.0002	0.0002
Thiamine • HCl	0.0002	0.0002
Vitamin B₁₂	0.002	0.002
Other
Peptone	0.6	0.6
D-Glucose	3	3
Glutathione (reduced)	0.0005	0.0005
Phenol Red • Na	0.011	0.011
Add
Sodium Bicarbonate	2.2	—
L-Glutamine	—	0.21915