These products are available in the US, Canada, and select European countries.
New skeletal muscle cells originate from quiescent satellite cells, which reside in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes. In studying muscle damage, primary human skeletal muscle cells (SkMCs) have been proven useful in elucidating mechanisms of muscle regeneration and differentiation, as well as in modeling neuromuscular disorders and disease.
PromoCell® Primary Human Skeletal Muscle Cells are produced from normal human skeletal muscle tissue from different locations, including M. pectoralis major or M. gluteus (lot specific source information is available on request). These cells can be induced to differentiate into multinucleated syncytia using PromoCell® Skeletal Muscle Cell Differentiation Medium. Shortly after isolation, all human skeletal muscle cells are cryopreserved at passage 2 (P2) using a proprietary, serum-free freezing medium, Cryo-SFM (C-29910). Each cryovial contains at least 500,000 viable cells after thawing. Human Skeletal Muscle Cells are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days.
The Skeletal Muscle Cell Growth Medium is a low-serum medium developed for the expansion of human skeletal muscle cell cultures. The Skeletal Muscle Cell Differentiation Medium is a serum-free medium developed to induce the fusion of skeletal muscle cells to myotubes with typical multinucleated syncytia. These media are optimized for growing primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells. The Skeletal Muscle Cell Growth Medium is available in two formats: “ready-to-use” or “kit”. The “ready-to-use” option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Skeletal Muscle Cell Growth Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements), giving the user full control over the media formulation. The SupplementMix or SupplementPack is added to Basal Medium to prepare the complete Skeletal Muscle Cell Medium. Basal Medium (with or without phenol red), SupplementMix, and the SupplementPack are also available for individual purchase.
Skeletal Muscle Cell Growth Medium contains all the growth factors and supplements necessary for the optimal growth of human skeletal muscle cells, including epidermal growth factor, basic fibroblast growth factor, and insulin (Table 1). Skeletal Muscle Cell Differentiation Medium is a serum-free medium containing insulin. These media do not contain antibiotics or antimycotics and are formulated for use in an incubator with an atmosphere of 5% CO2
Rigid quality control (QC) tests are performed for each lot of Human Skeletal Muscle Cells.
All lots of the Skeletal Muscle Cell Growth Medium are subjected to rigorous and comprehensive quality control tests using primary human SkMC. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the SkMC used for testing. Additionally, the Skeletal Muscle Cell Differentiation Medium is validated for the capacity to fully induce the differentiation of SkMC. Approved in-house lots of Skeletal Muscle Cell Media are used as a reference.
All lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma). For safety precautions, please refer to the appropriate safety data sheet (SDS).
Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for serum-based trypsin neutralization (as when subculturing the cells). We recommend instead using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
Intended Use of Primary Human Skeletal Muscle Cells and Keratinocyte Growth Media
Primary Human Skeletal Muscle Cells and Skeletal Muscle Cell Growth Media are for in vitro use only and not for diagnostic or therapeutic procedures. For safety information, please see appropriate SDS.
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