Figure 1.CRISPRi and CRISPRa utilize catalytically inactive dCas9 to recruit the transcriptional repressor KRAB domain or the transcriptional activators HSF1, p65 and MS2 to silence and overexpress target gene expression respectively.
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The Sigma-Aldrich® CRISPR inhibition libraries have superior algorithm placement and improved sgRNA scaffolding for optimal gene knockdown, even in the most difficult targets.
Discover our optimized CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Our pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized. Individual guide RNAs for both systems are also easily ordered online.
Sigma-Aldrich® Advanced Genomics CRISPRi and CRISPRa libraries complement our comprehensive suite of genome engineering technologies to allow you to uncover further insights into complex biological processes. Large scale LOF (loss-of-function) and GOF (gain-of-function) screens can identify unique, yet functionally related, gene pathways and hits which are often missed under otherwise identical KO (knockout) screening parameters. Take your experiments Beyond the Bench with the largest genomics portfolio available and a partner that can ensure your experimental goals are met.
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