CRISPRi and CRISPRa utilize catalytically inactive dCas9 to recruit the transcriptional repressor KRAB domain or the transcriptional activators HSF1, p65 and MS2 to silence and overexpress target gene expression respectively.

Figure 1.CRISPRi and CRISPRa utilize catalytically inactive dCas9 to recruit the transcriptional repressor KRAB domain or the transcriptional activators HSF1, p65 and MS2 to silence and overexpress target gene expression respectively.

Not sure which screening tool is right for your experiment? Pricing questions? Need a custom pool? We can help!

Resources

Screening Tips Video

The Sigma-Aldrich® CRISPR inhibition libraries have superior algorithm placement and improved sgRNA scaffolding for optimal gene knockdown, even in the most difficult targets.

Added Benefits:

Vector Maps

SAM CRISPRa

LV06
LV07
SAM CRISPRa Helper Construct-1 dCas9 VP64 Blasticidin
SAM CRISPRa Helper Construct 2 MS2-P65-HSF1-Hygromycin

CRISPRi

LV17
KRAB-dCas9-2A-Blast

Lentiviral Libraries and Pools for CRISPR Activation and Inhibition

Discover our optimized CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Our pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized. Individual guide RNAs for both systems are also easily ordered online.

Sigma-Aldrich® Advanced Genomics CRISPRi and CRISPRa libraries complement our comprehensive suite of genome engineering technologies to allow you to uncover further insights into complex biological processes. Large scale LOF (loss-of-function) and GOF (gain-of-function) screens can identify unique, yet functionally related, gene pathways and hits which are often missed under otherwise identical KO (knockout) screening parameters. Take your experiments Beyond the Bench with the largest genomics portfolio available and a partner that can ensure your experimental goals are met.

Specifications

CRISPRi Available Libraries and Pools

SAM CRISPRa Libraries and Helper Constructs

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