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Custom DNA and RNA Oligos & qPCR Probes Product Resources

Featured Articles and Protocols

  • Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
  • We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of Tm.
  • Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
  • Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. Heat breaks all hydrogen bonds, and cooling allows new bonds to form between the sequences.

Technical Literature

  • Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
  • This technical bulletin will help you select the best purification option for your oligo, depending upon the oligo type and its intended application.
  • DNA absorbs ultraviolet light due to its highly conjµgated nature. DNA may thus be easily quantitated in a UV spectrometer.
  • Several phosphate backbone variants have been developed in an attempt to alter the chemical properties of native-state DNA and therefore overcome the two major challenges involved with using oligonucleotides in vivo.
  • The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular nuclease degradation.
  • There is a wide selection of possible custom probes that can be used for qPCR, and each has advantages for different applications.
  • Digital PCR (dPCR) is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences.
  • Frequently asked questions about Locked Nucleic Acids (LNA)
  • Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background because of its own native fluorescence. DABCYL, though a dark quencher, has an inadequate absorption footprint that overlaps very poorly with reporter dyes emitting above 480 nm.
  • Oligonucleotide Handling & Stability is a techniques in order to ensure trouble-free experiments. Proper storage of your oligo will maximize its shelf life, allowing you to get the most use from the oligo
  • Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free in solution.

Protocols, Procedures, Safety & Quality



Product Citations & Our Publications

1.
Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker, Jr. JR, Norris TB. 2010. Real-Time Biomolecular Binding Detection Using a Sensitive Photonic Crystal Biosensor. Anal. Chem.. 82(12):5211-5218. http://dx.doi.org/10.1021/ac100576y
2.
Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. http://dx.doi.org/10.1126/science.1170160
3.
Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. http://dx.doi.org/10.1371/journal.pone.0008291
4.
Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. http://dx.doi.org/10.1021/ja106158b
5.
Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. http://dx.doi.org/10.1038/nature08611
6.
Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. http://dx.doi.org/10.1073/pnas.1015858107
7.
Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. http://dx.doi.org/10.1186/gb-2007-8-10-r227
8.
Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. http://dx.doi.org/10.1093/nar/gkp802
9.
Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. http://dx.doi.org/10.1093/nar/gkq1196