Custom DNA and RNA Oligos & qPCR Probes Product Resources
Featured Articles and Protocols
- Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
- We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of Tm.
- Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
- Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. Heat breaks all hydrogen bonds, and cooling allows new bonds to form between the sequences.
Technical Literature
- Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
- This technical bulletin will help you select the best purification option for your oligo, depending upon the oligo type and its intended application.
- DNA absorbs ultraviolet light due to its highly conjµgated nature. DNA may thus be easily quantitated in a UV spectrometer.
- Several phosphate backbone variants have been developed in an attempt to alter the chemical properties of native-state DNA and therefore overcome the two major challenges involved with using oligonucleotides in vivo.
- The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular nuclease degradation.
- There is a wide selection of possible custom probes that can be used for qPCR, and each has advantages for different applications.
- Digital PCR (dPCR) is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences.
- Frequently asked questions about Locked Nucleic Acids (LNA)
- Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background because of its own native fluorescence. DABCYL, though a dark quencher, has an inadequate absorption footprint that overlaps very poorly with reporter dyes emitting above 480 nm.
- Oligonucleotide Handling & Stability is a techniques in order to ensure trouble-free experiments. Proper storage of your oligo will maximize its shelf life, allowing you to get the most use from the oligo
- Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free in solution.
Protocols, Procedures, Safety & Quality
- This protocol is for conjugating NHS-ester modifications to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
- Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. Heat breaks all hydrogen bonds, and cooling allows new bonds to form between the sequences.
- Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides, thereby avoiding this source of oligo dimer formation.
- Safety Data Sheets for Oligonucleotides.
- The foundation of Sigma's manufacturing process is a robust Quality Management System, which drives compliance to ISO 9001:2008 certification.
- We are committed to product quality, manufacturing effectiveness and meeting customer expectations. Accordingly, the majority of our Life Science sites are certified to the ISO 9001:2015 standard.
Product Citations & Our Publications
1.
Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker, Jr. JR, Norris TB. 2010. Real-Time Biomolecular Binding Detection Using a Sensitive Photonic Crystal Biosensor. Anal. Chem.. 82(12):5211-5218. http://dx.doi.org/10.1021/ac100576y
2.
Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. http://dx.doi.org/10.1126/science.1170160
3.
Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. http://dx.doi.org/10.1371/journal.pone.0008291
4.
Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. http://dx.doi.org/10.1021/ja106158b
5.
Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. http://dx.doi.org/10.1038/nature08611
6.
Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. http://dx.doi.org/10.1073/pnas.1015858107
7.
Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. http://dx.doi.org/10.1186/gb-2007-8-10-r227
8.
Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. http://dx.doi.org/10.1093/nar/gkp802
9.
Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. http://dx.doi.org/10.1093/nar/gkq1196
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Josefsen MH, Löfström C, Sommer HM, Hoorfar J. 2009. Diagnostic PCR: Comparative sensitivity of four probe chemistries. Molecular and Cellular Probes. 23(3-4):201-203. http://dx.doi.org/10.1016/j.mcp.2009.02.003
2.
Saito Y, Kitamura H, Hijikata A, Tomizawa-Murasawa M, Tanaka S, Takagi S, Uchida N, Suzuki N, Sone A, Najima Y, et al. 2010. Identification of Therapeutic Targets for Quiescent, Chemotherapy-Resistant Human Leukemia Stem Cells. Science Translational Medicine. 2(17):17ra9-17ra9. http://dx.doi.org/10.1126/scitranslmed.3000349
1.
Schutkowski A, König B, Kluge H, Hirche F, Henze A, Schwerdtle T, Lorkowski S, Dawczynski C, Gabel A, Große I, et al. 2019. Metabolic footprint and intestinal microbial changes in response to dietary proteins in a pig model. The Journal of Nutritional Biochemistry. 67149-160. http://dx.doi.org/10.1016/j.jnutbio.2019.02.004
2.
Schutkowski A, Max D, Bönn M, Brandsch C, Grundmann SM, Hirche F, Staege MS, Stangl GI. 2018. Vitamin D Does Not Play a Functional Role in Adipose Tissue Development in Rodent Models. Mol. Nutr. Food Res.. 62(4):1700726. http://dx.doi.org/10.1002/mnfr.201700726
3.
Kühne H, Hause G, Grundmann SM, Schutkowski A, Brandsch C, Stangl GI. 2016. Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression. Nutrition Research. 36(2):184-192. http://dx.doi.org/10.1016/j.nutres.2015.10.005
4.
Schutkowski A, Wege N, Stangl GI, König B. Tissue-Specific Expression of Monocarboxylate Transporters during Fasting in Mice. PLoS ONE. 9(11):e112118. http://dx.doi.org/10.1371/journal.pone.0112118
5.
Kühne H, Schutkowski A, Weinholz S, Cordes C, Schierhorn A, Schulz K, König B, Stangl GI. 2014. Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response. Lipids Health Dis. 13(1):51. http://dx.doi.org/10.1186/1476-511x-13-51
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Hudnall SD, Chen T, Allison P, Tyring SK, Heath A. 2008. Herpesvirus prevalence and viral load in healthy blood donors by quantitative real-time polymerase chain reaction. Transfusion. 48(6):1180-1187. http://dx.doi.org/10.1111/j.1537-2995.2008.01685.x
*qPCR primers and probes were used to detect virus in blood samples; a Custom Products scientist co-authored this paper.