Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Choose Dual-Labeled Probes for:

  • Microarray validation
  • Multiply target genes / few samples
  • Pathogen detection
  • Multiplexing
  • Viral load quantification
  • Gene expression analysis
  • Gene copy determination

Perfect for validating MISSION® siRNA and shRNA knockdowns.

Benefits of Using Dual-Labeled Probes include:

  • Design simplicity for sequence specificity
  • Extensive availability of reporter/quencher combinations
  • Increased sensitivity

Add Locked Nucleic Acid to Your Probe for:

  • Increased thermal stability and hybridization specificity
  • Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
  • Easier and more sensitive probe designs for problematic target sequences

How Dual-Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3’ end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism.

Dual-Labeled Probes Work

The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Product Features include:

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Custom formats available (normalization, special plates, etc.)

Our probes are provided in a format to simplify your experimental planning.

Guaranteed Yields

*Estimate is based on 4 nmoles or 32 µg for 1 OD and 200 nM in 25 µL reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 25 bases.

Spectral Properties Table

1JOE/TET alternative
2VIC® alternative
3Cyanine 3 alternative
4TAMRA™ alternative
5Cyanine 5 alternative

Shipping Schedule

*Delivery times may be longer due to international transit, customs clearance delays, etc. Large projects will be placed on a delivery schedule based upon project needs.
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IMPORTANT UPDATE

Beginning May 8, 2022, BHQ™ (Black Hole Quencher™) 1 and 2 will no longer be available as a modification to Custom DNA Oligos and Custom qPCR Probes. The replacements will be BXQ™ (Block All Quencher™) 1 and 2. Information about the BXQ dark quenchers will be added from time-to-time to key oligo landing pages up until the date of change. As these key landing pages are updated, this message will be updated to provide links to the new information. If you have any questions, please contact your local company representative.

Alternatively, to learn more about BXQ, please visit the manufacturer’s website: BXQ | AAT Bioquest.