Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Perfect for validating MISSIONĀ® siRNA and shRNA knockdowns.
A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5ā end and a quencher molecule located at the 3ā end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism.
The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.
Our probes are provided in a format to simplify your experimental planning.
Guaranteed Yields |
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Spectral Properties Table |
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1JOE/TET alternative
2VICĀ® alternative
3Cyanine 3 alternative
4TAMRA⢠alternative
5Cyanine 5 alternative
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