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Microbiological Media and Detection

Chloe McClanahan

HybriScan™ Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. We provide a revolutionary molecular biology method that is rapid, easy, and cost effective. Based on the detection of rRNA, this method completely avoids the need for PCR amplification. The sandwich hybridization test, called HybriScan, is performed on a microtiter plate. The range of lactobacilli detected by HybriScan™ tests is listed in the table. 

NameApplicationReactionsCat. No.
HybriScan™ D BeerDetection of all relevant beer spoilage organisms of genus Lactobacillus, Pediococcus, Pectinatus and Megasphaera96 reactions62533
HybriScan™ D DrinksDetection of bacteria and yeasts in fruit juices and nonalcoholic beverage; determination of total viable count96 reactions68301
HybriScan™ D LactobacDetection of Lactobacilli in fruit juices and non-alcoholic beverage.96 reactions59744
HybriScan™ I Lactobacillus brevisIdentification of Lactobacillus brevis48 reactions75724
HybriScan™ I Lactobacillus buchneriIdentification of Lactobacillus buchneri48 reactions80065
HybriScan™ I Lactobacillus lindneriIdentification of Lactobacillus lindneri48 reactions86827

Selective Media for Bifidobacteria

NameApplicationCat. No.
BSM AgarFor the selective isolation and identification of Bifidobacteria.
The medium is used for quality control in the manufacture of dairy products.
88517
BSM BrothFor the selective isolation and identification of Bifidobacteria.
The medium is used for quality control in the manufacture of dairy products.
90273
BSM SupplementAn antimicrobial supplement recommended for the selective isolation of Bifidobacteria.83055
Wilkins Chalgren anaerobic agarUsed for the isolation of anaerobic bacteria.W1761

Differentiation Media for Lactobacilli

NameApplicationCat. No.
Litmus MilkFor maintenance of Lactobacilli and for determining the action of bacteria on milk.17158
LS Diff erential AgarFor the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, 2,3,5-triphenyltetrazolium chloride reduction, and casein reaction.17153
WL Nutrient AgarFor the examination of materials encountered in brewing and for industrial fermentations containing mixed fl ora of yeasts and bacteria.17222
WL nutrient brothRecommended for the cultivation of bacteria encountered in breweries and industrial fermentations.W2261

Nonselective Media for Lactobacilli

NameApplicationCat. No.
Elliker BrothFor culturing Streptococci and Lactobacilli of importance in the dairy industry.17123
Plate Count Skim Milk AgarFor the enumeration of bacteria in milk and dairy products.80957
Tomato Juice AgarFor the cultivation and enumeration of Lactobacilli.17216
Tomato Juice BrothFor cultivation of yeasts and other aciduric microorganisms.17218
Tryptone Glucose Yeast Extract AgarRecommended for enumeration of bacteria in water, air, milk and dairy products.T2188
Yeast malt agarUsed for the isolation and cultivation of yeasts, molds and other aciduric microorganisms.Y3127

Selective Media (by low pH and selective agents) for Lactobacilli

NameApplicationCat. No.
Lactobacillus bulgaricus Agar (Base)Used with acetate buffer for isolation and identification of Lactobacillus bulgaricus.17154
MRS AgarFor the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe.69964
MRS Agar, original acc. DeMan-Rogosa-SharpeFor the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of materials. Recommended by the "Schweizerisches Lebensmittelbuch" 5th ed., chapter 56A.30912
MRS Agar, VegitoneThis MRS Agar is free of animal-derived material. It is recommended for the isolation and cultivation of Lactobacillus species.41782
MRS BrothFor the enrichment and isolation of all species of Lactobacilli from all types of material.69966
MRS Broth modifi ed, VegitoneThis MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species.38944
NBB Agar Base, modifiedSelective medium used for the detection of contaminating/spoilage microorganisms in brewery.64198
NBB Broth Base, modifiedSelective medium used for the detection of contaminating/spoilage microorganisms in brewery.50725
Orange-serum AgarFor the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit, according to Hays, Troy, and Beisel. The prepared agar may be turbid after autoclaving due to orange extract.75405
Raka-Ray AgarA medium for selective isolation of lactic acid bacteria from beer and brewing processes02538
Rogosa SL agarUsed as a selective medium for cultivation of Lactobacilli.R1148
Sorbic acid Agar (Base)For the isolation and differentiation of Lactobacilli from food, feces, etc. according to Reuter.85515
Universal Beer AgarFor culturing microorganisms of significance in the brewing industry.17226
WL Diff erential AgarFor selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.17215
Additional Materials
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